Monkey Tumor Necrosis Factor Related Apoptosis Inducing Ligand 3 (TRAIL-R3) Enzyme-Linked Immunosorbent Assay (ELISA) Kit Instructions for Use

This reagent is for research use only: This kit is used to determine the content of tumor necrosis factor-related apoptosis-inducing ligand 3 (TRAIL-R3) in monkey serum, plasma and related liquid samples. Experimental principle: This kit uses the double antibody sandwich method to determine the level of TNF-related inhibitory ligand 3 (TRAIL-R3) in monkeys. The purified mouse TNF-related apoptosis-inducing ligand 3 (TRAIL-R3) antibody was coated with a microplate to prepare a solid phase antibody, and the tumor necrosis factor-related apoptosis-inducing compound was sequentially added to the microcapsules of the coated monoclonal antibody. Body 3 (TRAIL-R3), which binds to HRP-labeled tumor necrosis factor-related apoptosis-inducing ligand 3 (TRAIL-R3) antibody to form an antibody-antigen-enzyme-labeled antibody complex. After thorough washing, substrate TMB is added. Color development. TMB is converted to blue under the catalysis of HRP enzyme and converted to the final yellow color by the action of an acid. The color depth is positively correlated with tumor necrosis factor-related apoptosis-inducing ligand 3 (TRAIL-R3) in the sample. The absorbance (OD value) was measured by a microplate reader at a wavelength of 450 nm, and the content of monkey tumor necrosis factor-related apoptosis-inducing ligand 3 (TRAIL-R3) in the sample was calculated by a standard curve. Kit composition: Kit composition 48-well configuration 96-well configuration Storage instructions 1 part 1 part sealing film 2 pieces (48) 2 pieces (96) Sealed bag 1 1 enzyme label coated plate 1 × 48 1 × 96 2 -8 ° C preservation standard: 180ng / L 0.5ml × 1 bottle 0.5ml × 1 bottle 2-8 ° C preservation standard dilution 1.5ml × 1 bottle 1.5ml × 1 bottle 2-8 ° C preservation enzyme standard reagent 3 ml × 1 bottle 6 ml × 1 bottle 2-8 ° C Preservation sample dilution 3 ml × 1 bottle 6 ml × 1 bottle 2-8 ° C Preservation developer A solution 3 ml × 1 bottle 6 ml × 1 bottle 2-8 ° C preservation Reagent B liquid 3 ml × 1 bottle 6 ml × 1 bottle 2-8 ° C Preservation solution 3 ml × 1 bottle 6 ml × 1 bottle 2-8 ° C Preservation concentrated washing solution (20ml × 20 times) × 1 bottle (20ml × 30 times) × 1 bottle 2-8 ° C preservation

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